NewAgrobacterium helper plasmids for gene transfer to plants |
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| Authors: | Elizabeth E. Hood Stanton B. Gelvin Leo S. Melchers Andre Hoekema |
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| Affiliation: | (1) Department of Biology, Utah State University, 84322-5305 Logan, UT, USA;(2) Department of Biological Sciences, Purdue University, 47907 West Lafayette, IN, USA;(3) MOGEN International, Einsteinweg 97, 2333 CB Leiden, Netherlands |
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| Abstract: | We describe the construction of new helper Ti plasmids forAgrobacterium-mediated plant transformation. These plasmids are derived from three differentAgrobacterium tumefaciens Ti plasmids, the octopine plasmid pTiB6, the nopaline plasmid pTiC58, and the L,L-succinamopine plasmid pTiBo542. The T-DNA regions of these plasmids were deleted using site-directed mutagenesis to yield replicons carrying thevir genes that will complement binary vectorsin trans. Data are included that demonstrate strain utility. The advantages ofAgrobacterium strains harbouring these  disamed Ti plasmids for plant transformation viaAgrobacterium are discussed. |
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| Keywords: | Agrobacterium plant transformation helper plasmids host range Ti plasmid T-DNA |
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