|
|||||
|
|
Expression and self-assembly of norwalk virus capsid protein from venezuelan equine encephalitis virus replicons |
|
| Authors: | Baric Ralph S Yount Boyd Lindesmith Lisa Harrington Patrick R Greene Shermalyn R Tseng Fan-Chen Davis Nancy Johnston Robert E Klapper David G Moe Christine L |
| Institution: | Department of Epidemiology, Program in Infectious Diseases, School of Public Health, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7400, USA. rbaric@sph.unc.edu |
| Abstract: | The Norwalk virus (NV) capsid protein was expressed using Venezuelan equine encephalitis virus replicon particles (VRP-NV1). VRP-NV1 infection resulted in large numbers of recombinant NV-like particles that were primarily cell associated and were indistinguishable from NV particles produced from baculoviruses. Mutations located in the N-terminal and P1 domains of the NV capsid protein ablated capsid self-assembly in mammalian cells. |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ | |