Neuronal protein gene product 9.5 (IEF SSP 6104) is expressed in cultured human MRC-5 fibroblasts of normal origin and is strongly down-regulated in their SV40 transformed counterparts |
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Authors: | Bent Honor Hanne H Rasmussen Joël Vandekerckhove Julio E Celis |
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Institution: | Institute of Medical Biochemistry, Aarhus University, Denmark. |
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Abstract: | Neuronal protein gene product 9.5 (PGP 9.5) most likely identical to ubiquitin carboxyl-terminal hydrolase isozyme LI (UCH-LI) has been reported to be expressed almost exclusively in neuronal and neuroendocrine tissues. By two-dimensional (2D) immunoblotting, comigration and microsequencing of proteins recovered from 2D gels we have identified PGP 9.5/UCH-LI as polypeptide IEF SSP 6104 (Mr = 27000, PL = 5.49) in the comprehensive 2D gel cellular protein database of human embryonal lung MRC-5 fibroblasts (1989) Electrophoresis 10, 76–115; (1990) Electrophoresis 11, 1072–1113]. This protein is expressed at high levels in quiescent and proliferating cultured normal fibroblasts and is strongly down-regulated (about 10 times) in their transformed counterparts. |
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Keywords: | Normal human fibroblasts SV40 transformed human fibroblast PGP 9 5 UCH-LI Transformation sensitive protein 2D-gel electrophoresis |
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