Histochemical Identification of Basic Proteins with Biebrich Scarlet at Alkaline pH |
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| Authors: | S S Spicer R D Lillie |
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| Institution: |
a Laboratory of Pathology and Histochemistry, National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda 14, Maryland
b Department of Pathology, Louisiana State University School of Medicine, New Orleans 12, Louisiana |
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| Abstract: | Staining at graded alkaline pH levels with the sulfonated dye, Biebrich scarlet, shows basic proteins in various histologic sites, and differentiates the sites according to their relative basicity. Certain structures stain at pH 6.0 but not at 8.0 or above. Others stain maximally up to pH 93 and a few stain strongly as high as pH 103. The most strongly basic sites resist more than the others the destruction of acidophilia by nitrosation, acetylation or exposure to formaldehyde. |
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