Use of haloacetate dehalogenase genes as selection markers forEscherichia coli andPseudomonas vectors |
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| Authors: | Haruhiko Kawasaki Hidetoshi Kuriyama Kenzo Tonomura |
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| Institution: | (1) Department of Agricultural Chemistry, University of Osaka Prefecture, 593 Sakai City, Osaka, Japan |
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| Abstract: | The haloacetate dehalogenase gene,dehH2, cloned fromMoraxella sp. strain B could be used as a selection marker gene for vectors inEscherichia coli andPseudomonas putida. Haloacetates, especially iodoacetate, inhibit the growth of some microorganisms. ThedehH2 gene introduced into the cells conferred iodoacetate resistance on them. Therefore,E. coli andP. putida transformed with vectors marked withdehH2 could be easily selected on plates containing iodoacetate. |
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| Keywords: | haloacetate dehalogenase iodoacetate resistance dehalogenase gene |
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