Production of culmorin compounds and other secondary metabolites by Fusarium culmorum and F. graminearum strains isolated from Norwegian cereals |
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Authors: | Langseth Wenche Ghebremeskel Mebrat Kosiak Barbara Kolsaker Per Miller David |
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Institution: | (1) Dept. Feed and Food Hygiene, National Veterinary Institute, N-0033 Oslo, Norway;(2) Dept. Chemistry, University of Oslo, N-0315 Oslo, Norway;(3) Ottawa-Carleton Institute of Chemistry, Carleton University, Ottawa, Ontario, K1S 5B6, Canada |
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Abstract: | Twenty-three Fusarium culmorum and 21 F. graminearumisolates were studied for their ability to produce mycotoxins and other secondary metabolites. The strains were cultivated
on rice, and the extracts analysed by gas chromatography mass spectrometry (GC-MS) after derivatization with pentafluoropropionic
(PFP) reagent. Two F. culmorum strains formed nivalenol and its acetylated derivatives (chemotype II), while all F. graminearum and the otherF. culmorum isolates produced deoxynivalenol (DON) via 3-acetyldeoxynivalenol (3-acetyl-DON) (chemotype IA). 15-hydroxy-culmorin, followed
by 5-hydroxy-culmorin were the main other metabolites produced F. culmorum, while 5-, 12- and an unidentified hydroxy-culmorin, suggested to be 14-hydroxy-culmorin, were the main metabolites of F. graminearum. The hydroxy-culmorin profile was found to be significantly different for the two Fusarium species. Minor amounts of about ten other hydroxy-culmorins, four hydroxy-culmorones and 3,13-dihydroxy-epiapotrichothecene
were also detected in most cultures. Traces of sambucinol seemed to be present in some of the isolates, but were not detected
in any significant amounts. The precursors in the biosynthetic sequence to 3-acetyldeoxynivalenol,7,8-dihydroxycalonectrin
and 15-deacetyl-7,8-dihydroxycalonectrin,were detected in most cultures. We also report the assignment of both the 1H and13C NMR data of 15-deacetyl-7,8-dihydroxycalonectrin, which has only been reported incorrectly before.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | 15-deacetyl-7 8-dihydroxycalonectrin gas chromatography hydroxy-culmorone mass spectrometry hydroxy-culmorin sambucinol |
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