Identifying Chinese species of Gammarus (Crustacea: Amphipoda) using DNA barcoding |
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作者单位: | Zhong-e HOU,Shu-qiang LI(Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China);Zhu LI(Beijing Museum of Natural History,Beijing 100050,China)
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基金项目: | 国家自然科学基金,Beijing Financial Budget Index,北京市自然科学基金 |
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摘 要: |
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关 键 词: | 动物学 甲壳纲动物 研究 进展 DNA |
Identifying Chinese species of Gammarus(Crustacea:Amphipoda) using DNA barcoding |
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Authors: | Zhong-e HOU Zhu LI Shu-qiang LI |
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Institution: | [1]Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China [2]Beijing Museum of Natural History, Beijing 100050, China |
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Abstract: | Using a standard cytochrome c oxidase I sequence, DNA barcoding has been shown to be effective to distinguish known species and to discover cryptic species. Here we assessed the efficiency of DNA barcoding for the amphipod genus Gammarus from China. The maximum intraspecific divergence for widespread species, Gammarus lacustris, was 3.5%, and mean interspecific divergence reached 21.9%. We presented a conservative benchmark for determining provisional species using maximum intraspecific divergence of Gammarus lacustris. Thirty-one species possessed distinct barcode clusters. Two species were comprised of highly divergent clades with strong neighbor-joining bootstrap values, and likely indicated the presence of cryptic species. Although DNA barcoding is effective, future identification of species of Gammarus should incorporate DNA barcoding and morphological detectionCurrent Zoology 55(2):158-164,2009]. |
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Keywords: | Gammarus Cytochrome c oxidase I DNA barcoding Cryptic species Taxonomy |
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