2型糖尿病兼抑郁症大鼠模型的建立与评价

目的：用体重检测、空腹血糖检测、宏观表征、旷场实验行为学评价糖尿病兼抑郁症的大鼠模型。方法采用高脂饲料喂养加腹腔注射小剂量链脲佐菌素（ STZ）的方法制备2型糖尿病模型，在其基础上再用21 d慢性束缚的方法建立糖尿病兼抑郁症大鼠模型。将32只Wistar大鼠随机分为3组（ n ＝8）：正常组（N组），2型糖尿病组（T组），2型糖尿病兼抑郁症组（T＋D组）。2型糖尿病模型建立后，在慢性束缚的第0、7、14、21天检测大鼠的空腹血糖和体重，并对大鼠的宏观表征、饮食量、粪便、小便、精神状态进行观察，在第21天利用行为学设备分析软件，对大鼠旷场实验进行分析，检测大鼠的抑郁程度，验证评价2型糖尿病兼抑郁症大鼠模型是否成功。结果给予高脂饲料及腹腔注射STZ制备2型糖尿病模型后，T＋D组大鼠的毛发散乱，无光泽，活动迟缓，进食量、饮水量增加，粪便尿量增加，精神萎靡。第0、7、14、21天T组和T＋D组组大鼠体重均下降，与N组比较差异有显著性（P＜0.05；P＜0.01），21d慢性束缚刺激后，T＋D组体重比T组大鼠体重增加较慢，差异有显著性（P＜0.05）；第0、7、14、21天，T组和T＋D组大鼠血糖均升高，与N组比较差异有显著性（ P＜0.01），21 d慢性束缚刺激后，第21天T＋D组大鼠血糖比T组较高，差异有显著性（P＜0.01），大鼠5 min内总移动距离有变化，与N组相比，T组差异没有显著性（P＞0.05），T＋D组差异有显著性（P＜0.05）；与N组相比，T组大鼠5 min内移动速度减慢，差异有显著性（P＜0.05），T＋D组差异有显著性（ P＜0.01）。结论利用高脂饲料喂养加腹腔注射小剂量STZ及21天慢性束缚的方法，可以成功复制2型糖尿病兼抑郁症大鼠模型，适用于后续研究。

Establishment and evaluation of a rat model of type 2 diabetes associated with depression

Objective To evaluate the rat model of type 2 diabetes mellitus （ T2DM） associated with depres-sion by body weight, fasting blood glucose, manifestations, and open field test.Methods The T2DM rat model was induced by high fat diet and low dose of STZ injection, and in addition, the T2DM rats were made restraint stress for 21 days.32 Wistar rats were randomly divided into three groups： normal group （ group N） , T2DM group （ group T）and T2 DM with depression group （ group T ＋D） , with 8 rats in each group.After the model was established, to measure the body weight, fasting blood glucose at day 0, 7, 14, and 21, and observe the gross manifestations, drink-ing and diet, feces, urine, and mental state, and test the rat depression by open field test.Results After establish-ment of the T＋D rat model, the rats in group T＋D showed some symptoms, including messy dark and gloomy hair, slow movement, increasing drinking, diet, feces and urine and mental fatigue.At day 0, 7, 14, and 21, compared with the group N, the body weight of the group T and group T＋D were decreased, showing a significant difference （ P＆lt;0.05;P＆lt;0.01）.At day 0, 7, 14, and 21, compared with the group N, and the fasting blood glucose in the groups T and T＋D were increased, with a significant difference （ P＆lt;0.01 ） .After 21 days of restraint stress, the fasting blood glucose in the group T＋D was significantly higher than that in the group T （ P＆lt;0.01 ） .Compared with the group N, the total movement distance in 5 minutes in the group T＋D was reduced, but without a significant differ-ence （1532.6 ＆#177;126.8 cm vs.940.5 ＆#177;208.3 cm, P＆gt;0.05）.Compared with the group N, the movement speed in 5 minutes in the group T was significantly slower than that in the group T , with a significant difference （ 5.1 ＆#177;0.4 cm/s vs.2.9 ＆#177;0.6 cm/s, P＆lt;0.05 ） , and even more slower than that in the group T ＋D, with a significant differ-ence （5.1 ＆#177;0.4 cm/s vs.2.4 ＆#177;0.