去卵巢大鼠血清和骨髓细胞碱性磷酸酶水平变化的动态观察

目的：动态观察去卵巢大鼠血清和骨髓细胞碱性磷酸酶（ ALP）水平的变化。方法将80只3月龄雌性SD大鼠按体重分层后随机分为基础组以及假手术和去卵巢3、6、12、24周组。分别在手术前（0）和手术后3、6、12、24周腹主动脉取血处死各组大鼠，分离血清，制备骨髓细胞甩片，用721分光光度计检测血清ALP水平的变化；用显微镜计数骨髓细胞甩片ALP阳性染色细胞的数目。结果在假手术组大鼠中，血清ALP水平在手术后3周显著上升并持续到手术后6周，但在手术后12周开始显著下降并持续到手术后24周；骨髓细胞ALP阳性染色细胞数目在手术后3周显著上升并持续到手术后12周，但在手术后24周却显著下降。在去卵巢组大鼠中，血清ALP水平在手术后3周显著上升，到手术后6周开始显著下降并一直持续到手术后24周；骨髓细胞ALP阳性染色细胞数目在手术后3周显著下降并持续到手术后24周。从手术后3周开始，去卵巢组大鼠血清ALP水平均显著高于假手术组大鼠，但骨髓细胞ALP阳性染色细胞数目均显著低于假手术组大鼠。结论假手术组大鼠血清和骨髓细胞ALP水平变化趋势基本相似，但去卵巢大鼠血清和骨髓细胞ALP水平变化趋势不同。

Dynamic changes of serum and bone marrow alkaline phosphatase levels in ovariectomized rats

Objective The aim of this study was to observe the dynamic changes of serum and bone marrow levels of alkaline phosphatase （ ALP） in ovariectomized （ OVX） rats.Methods Eighty 3-month-old non-pregnant female Spra-gue Dawley rats were randomly divided into following groups by body weight：baseline group, sham-operation （Sham）, and ovariectomized （OVX） rat groups.The rats were killed at 0, 3, 6, 12 or 24 weeks after operation.The changes of serum and bone marrow alkaline phosphatase （ ALP） levels were detected with photometer-721 and the number of ALP-positive bone marrow cells was detected with a research grade upright fluorescence microscope .Results In the sham group, the serum ALP levels were significantly increased in the weeks 3 until the week 6.However, the serum ALP levels were signifi-cantly decreased in the week 12 until the week 24.The ALP-positive cell number in the bone marrow was significantly in-creased in the week 3 until week 12, but significantly decreased in the week 24.In the OVX group, the serum ALP levels were significantly increased in the week 3, but significantly decreased in the week 6 until the week 24.The number of ALP-positive bone marrow cells was significantly decreased in the week 3 until the week 24.In addition, the serum ALP levels in the OVX group were significantly higher than that of the sham group .However, the number of ALP-positive bone marrow cells was significantly lower than that of the sham group .Conclusions The change tendency of serum ALP in the sham group is similar with that of bone marrow cells .But the change tendency of serum ALP in OVX rats is different from that of bone marrow cells .