Phenylpropanoid defence responses in transgenic Lotus corniculatus 1. Glutathione elicitation of isoflavan phytoalexins in transformed root cultures

Summary When Agrobacterium rhizogenes transformed root cultures of Lotus corniculatus were treated with glutathione, isoflavan phytoalexins accumulated in both tissue and culture medium. This accumulation of phytoalexins was preceded by a transient increase in the activity of phenylalanine ammonia lyase (PAL). Elicitation of PAL occurred throughout the growth curve of Lotus hairy roots and in different sectors of transformed root material.