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Stimulation of scion bud release by rol gene transformed rootstocks of Rosa hybrida L
Authors:van der Salm, T   Bouwer, R   van Dijk, A   Keizer, L   Hanisch ten Cate, C   van der Plas, L   Dons, J
Affiliation:Department of Developmental Biology, Centre for Plant Breeding and Reproduction Research (CPRO-DLO), PO Box 16, 6700 AA Wageningen, The Netherlands; Department Centre for Biometry Wageningen, Centre for Plant Breeding and Reproduction Research (CPRO-DLO), PO Box 16, 6700 AA Wageningen, The Netherlands; Department of Plant Physiology, Agricultural University Wageningen, PO Box 9101, 6700 HB Wageningen, The Netherlands; Corresponding author at: Florigene Europe b.v., Waardlaan 4A, 2231 NA Rijnsburg, The Netherlands
Abstract:For improvement of the root characteristics of roses, therolA, B andC genes from Agrobacteriumrhizogenes were introduced in the cultivar Moneyway, which isused as a rootstock. Four independent rolA+B+Ctransformants were produced. One rolC expressingtransformant, showing good growth and clearly decreased apical dominance,was selected for a grafting experiment with the cut rose cultivar Madelonas a scion. Grafting on this rolA+B+C transformedrootstock resulted in a stimulation of both root development of therootstock and axillary bud release of the untransformed scion. Axillary budrelease increased from 0.1 to 0.6 and from 0.3 to 1.3 basal shoots perplant at 15C and 20C, respectively. Since basal shoots from flowers,this altered plant architecture will presumably lead to a more efficientflower production.Keywords:Agrobacterium transformation, apicaldominance, rol genes, rootstock, rose.
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