Proteomics Analysis of Drought Stress-Responsive Proteins in <Emphasis Type="Italic">Hippophae rhamnoides</Emphasis> L. |
| |
Authors: | Gang Xu Chunyang Li Yinan Yao |
| |
Institution: | (1) Key Laboratory of Green Pesticide and Agricultural Bioengineering, Chinese Ministry of Education, Center for Research and Development of Fine Chemicals of Guizhou University, Guiyang, 550025, China;(2) Chengdu Institute of Biology, Chinese Academy of Sciences, P.O. Box 416, Chengdu, 610041, China;(3) China Graduate School of the Chinese Academy of Sciences, Beijing, 100039, China;(4) College of Agriculture, Guizhou University, Guiyang, 550025, China |
| |
Abstract: | Hippophae rhamnoides L. is uniquely capable of growing well under extreme environmental conditions such as water deficit, low temperature, and
high altitude. Such tolerance invokes much interest in understanding the biology of this plant species and its utilization
potential. In this study, analysis of drought stress-responsive proteins in H. rhamnoides was conducted wherein greenhouse-grown seedlings were subjected to drought stress. By using proteomic techniques, proteins,
extracted from leaves, were analyzed using two-dimensional electrophoresis and MALDI-TOF MS. Altogether, 55 proteins exhibited
changes in abundance under stress. Of these, 13 proteins were identified, including three that disappeared under drought (a
putative ABC transporter ATP-binging protein, a heat shock protein HslU, and a hypothetical protein XP-515578), seven that
were up-regulated (three large subunits of rubisco, a hypothetical protein DSM3645–23351, a putative acyl-CoA dehydrogenase,
a nesprin-2, and a J-type co-chaperone HSC20), and three that were only detected under drought (a probable nitrogen regulation
protein (NtrX), a 4-hydroxyphenylpyruvate dioxygenase, and an unnamed protein product). These proteins may function in β-oxidation
pathways in mitochondria, across membranes transport, abnormal protein removal, or prevent protein aggregation arrest, cell
division, cytoskeleton stabilization, iron–sulfur cluster assembly, nitrogen metabolism regulation, and antioxidant substance
biosynthesis. Four proteins (J-type co-chaperone Hsc20, a putative ABC transporter ATP-binging protein, NtrX, and HslU) were
deemed as new discoveries in higher plants, and their functions were predicted either from their conserved domains or homologies
to other organisms. These results provide new insights into our understanding of the mechanism of drought tolerance in plants. |
| |
Keywords: | Drought Two-dimensional gel electrophoresis Hippophae rhamnoides L Mass spectrometry |
本文献已被 SpringerLink 等数据库收录! |
|