Conversion of leukotriene C4 to leukotriene D4 by a cell-surface enzyme of rat macrophages |
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| Authors: | I Nagaoka T Yamashita |
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| Institution: | 1. Department of Allergy and Clinical Immunology, Ajou University School of Medicine, Suwon, South Korea;2. Department of Pharmacology, CKD Research institute, Yong-in, South Korea;3. Pediatric Department, Faculty of Medicine, University of Medicine and Pharmacy at Ho Chi Minh city, Vietnam;4. Department of Biomedical Science, Ajou University School of Medicine, Suwon, South Korea |
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| Abstract: | Leukotriene (LT) C4-metabolizing enzyme was studied using rat leukocytes. Neutrophils and lymphocytes hardly metabolized LTC4, whereas macrophages rapidly converted LTC4 to LTD4. The LTC4-metabolizing enzyme of macrophages was present in the membrane fraction but not in the nuclear, granular and cytosol fractions. When macrophages were modified chemically with diazotized sulfanilic acid, a poorly permeant reagent which inactivates cell-surface enzymes selectively, the LTC4-metabolizing activity of macrophages decreased significantly (greater than 90%). These findings suggest that rat macrophages possess the LTC4-metabolizing enzyme which converts LTC4 to LTD4, on the cell surface membrane. |
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