Genomic blot hybridization as a tool of phylogenetic analysis: Evolutionary divergence in the genusDrosophila |
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| Authors: | Michael Loukas Christos Delidakis Fotis C. Kafatos |
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| Affiliation: | (1) Department of Genetics, Agricultural College of Athens, Iera odos 75, 11855 Votanicos, Athens, Greece;(2) Department of Cellular and Developmental Biology, The Biological Laboratories, Harvard University, 16 Divinity Avenue, 02138 Cambridge, MA, USA;(3) Institute of Molecular Biology and Biotechnology, Department of Biology, University of Crete, 71110 Heraklio Crete, Greece |
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| Abstract: | Summary Comparative, quantitative Southern analysis of genomic DNA, using single-copy sequence probes, potentially is valuable for phylogenetic analysis. We have examined 27Drosophila species, belonging to two subgenera, seven species groups, and ten subgroups, using a variety of cloned and characterized probes: twelve cloned sequences fromD. melanogaster, two fromD. pseudoobscura, and two fromD. grimshawi. The data are generally congruent with accepted phylogenetic relationships inDrosophila, and confirm or clarify some previously uncertain relationships. The potential and limitations of the method are discussed.Presented at the FEBS Symposium on Genome Organization and Evolution, held in Crete, Greece, September 1–5, 1986 |
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| Keywords: | DNA divergence Quantitative Southern analysis Molecular phylogeny Drosophila evolution |
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