Virus-Like Particles from Escherichia coli-Derived Untagged Papaya Ringspot Virus Capsid Protein Purified by Immobilized Metal Affinity Chromatography Enhance the Antibody Response Against a Soluble Antigen |
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Authors: | Jesús Guerrero-Rodríguez Carlos Alberto Manuel-Cabrera Y Apatzingan Palomino-Hermosillo Paola Guadalupe Delgado-Guzmán Martha Escoto-Delgadillo Laura Silva-Rosales Sara Elisa Herrera-Rodríguez Carla Sánchez-Hernández Abel Gutiérrez-Ortega |
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Institution: | 1. Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Carretera Guadalajara-Nogales km 15.5, 45110, Zapopan, Jalisco, Mexico 2. Unidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Dise?o del Estado de Jalisco A.C., Normalistas 800, Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico 4. Unidad de Tecnología de Alimentos, Secretaría de Investigación y Posgrado, Universidad Autónoma de Nayarit, Boulevard Tepic-Xalisco S/N, 63155, Tepic, Nayarit, Mexico 3. Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Unidad Irapuato, Libramiento Norte km 9.6, Carretera Irapuato-León, 36821, Irapuato, Guanajuato, Mexico
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Abstract: | There is a growing interest in using virus-like particles (VLPs) as scaffolds for the presentation of antigens of choice to the immune system. In this work, VLPs from papaya ringspot virus capsid protein expressed in Escherichia coli were evaluated as enhancers of antibody response against a soluble antigen. Interestingly, although the capsid protein lacks a histidine tag, its purification by immobilized metal affinity chromatography was achieved. The formation of VLPs was demonstrated by electron microscopy for the first time for this capsid protein. VLPs were enriched by polyethylene glycol precipitation. Additionally, these VLPs were chemically coupled to green fluorescent protein in order to evaluate them as antigen carriers; however, bioconjugate instability was observed. Nonetheless, the adjuvant effect of these VLPs on BALB/c mice was evaluated, using GFP as antigen, resulting in a significant increase in anti-GFP IgG response, particularly, IgG1 class, demonstrating that the VLPs enhance the immune response against the antigen chosen in this study. |
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