Virus-Specific Ribonucleic Acid in the Nucleus and Cytoplasm of Rat Embryo Cells Transformed by Adenovirus Type 2 |
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| Authors: | Koichiro Shimada Kei Fujinaga Seiji Hama Kenji Sekikawa Yohei Ito |
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| Affiliation: | aLaboratory of Viral Oncology, Aichi Cancer Center, Research Institute, Chikusa-ku, Nagoya, Japan |
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| Abstract: | Nuclei were isolated from rat embryo cells transformed by adenovirus type 2. Nuclear and cytoplasmic virus-specific ribonucleic acids (RNA) were characterized and quantitated by deoxyribonucleic acid (DNA)-RNA hybrid formation with adenovirus DNA. The results indicate that most, if not all, virus-specific RNA molecules are synthesized in the cell nucleus and subsequently transported into cytoplasm where they degrade with a half-life of 1 to 2 hr. No difference in base sequences between nuclear and cytoplasmic virus-specific RNA species can be detected by hybridization competition experiment with viral DNA. |
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