Failure to cultivate rabies virus in vitro |
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Authors: | J. D. Verlinde A. Kret |
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Affiliation: | (1) Department of Bacteriology and Experimental Pathology of the Institute for Preventive Medicine, Leiden |
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Abstract: | Summary Report of the unsuccessful cultivation of rabies fixed virus in a cell-free medium consisting of steamed sheep-brain extract, glycine, peptone and a number of accessory factors (tryptophan, aneurine, nicotinic acid amide, pyridoxine hydrochloride, sodium pantothenate and lactoflavin). After 24 and 48 hour's incubation at 37° C, under aerobic conditions, the virus had completely lost its virulence, and did not induce immunity to reinoculation with active virus. |
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