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Amperometric biosensors based on dehydrogenase/NAD and heterocyclic quinones |
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| Authors: | L Geng LI Boguslavsky IP Kovalev SK Sahni H Kalash TA Skotheim HS Lee V Laurinavicius |
| Institution: | Moltech Corporation, 25 East Loop Road, Stony Brook, NY 11790-3350, USA Tel: [1] (520) 799 7558 Fax: [1] (520) 799 7501 Brookhaven National Laboratory, Building 480, Upton, NY 11973, USA Institute of Biochemistry, Mokslininku St. 12, Vilnius 2021, Lithuania |
| Abstract: | The electrocatalytic oxidation of NADH was evaluated from cyclic voltammetry with heterocyclic quinones dissolved in a water solution. The process of oxidation is not sensitive to the nature of the electrode surface and takes place on platinum, glassy carbon or carbon electrodes. NADH and glucose sensors constructed were investigated and operated at 0 mV vs. Ag/AgCl (in saturated KCl) using single potential step chronoamperometry. |
| Keywords: | biosensors glucose determination NADH electro-oxidation glucose dehydrogenase quinones |
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