Increased yield of fructose from glucose employing thermophilic xylose isomerase in water-ethanol mixtures |
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| Authors: | Kurt G. I. Nilsson Klaus Mosbach Kenji Sakaguchi |
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| Affiliation: | (1) Department of Pure and Applied Biochemistry, Chemical Center, University of Lund, P.O. Box 124, S-221 00 Lund, SWEDEN;(2) Present address: Department of Biotechnology, Chemical Center, University of Lund, P.O. Box 124, S-221 00 Lund, SWEDEN;(3) Present address: Nihon Shokuhin Kako Co., Ltd., 4-1 Marunouchi 3-chome, Chiyoda-ku, Tokyo, JAPAN |
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| Abstract: | Summary The isomerization of D-glucose in mixed ethanol-water was studied at various reaction temperatures (40–70 °C), employing glucose isomerase fromStreptomyces phaeochromogenes andClostridium thermohydrosulfuricum, respectively. The thermophilicClostridium enzyme was considerably, more stable towards the combination of organic cosolvent and increased temperature and with this enzyme a 55% yield of fructose from glucose was obtained at relatively low concentration of ethanol (40 %). |
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