Correction for non-uniform phosphate labeling of E. coli and phage RNAs synthesized in vivo

$\text{E. coli}$ cells grown to phosphate starvation incorporate ³²PO₄ unequally into the α position of the four ribonucleotide triphosphates during a short period of labeling. A method for determining the relative specific activities of nucleotides in RNA molecules synthesized under these conditions and correcting sequence data is described.