Inducible isopentenyl transferase as a high-efficiency marker for plant transformation. |
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Authors: | T Kunkel Q W Niu Y S Chan N H Chua |
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Affiliation: | Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Ave., New York, NY 10021-6399, USA. |
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Abstract: | Overexpression of the isopentenyltransferase gene (ipt) from the Ti-plasmid of Agrobacterium tumefaciens increases cytokinin levels, leading to generation of shoots from transformed plant cells. When combined with a dexamethasone-inducible system for controlling expression, ipt expression can be used to select for transgenic regenerants without using an antibiotic-resistance marker. The combined system allows efficient cointroduction of multiple genes (in addition to ipt) and produces transgenic plants without morphological or developmental defects. |
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