三叶半夏和掌叶半夏凝集素原核表达及特性研究

通过原核表达获得大量具有生物活性的三叶半夏和掌叶半夏凝集素，比较分析两种半夏凝集素的异同，并深入探讨半夏凝集素和亚基之间凝集活性的差异。结果表明，掌叶半夏凝集素的凝集活力为三叶半夏凝集素的4倍，凝集素第三个活性位点两个氨基酸的差异可能是引起三叶半夏和掌叶半夏凝集素凝集活性不同甚至药理作用不同的主要原因。原核表达系统获得的凝集素亚基不能凝集兔血红细胞。该研究为深入探讨两种半夏凝集素的区别，及半夏凝集素和亚基之间凝集活性的差异打下了基础，也为解决半夏资源紧缺提供了一个可能的方法。

Prokaryotic Expression and Characteristics Reseach of Pinellia Ternate and Pinellia Pedatisecta Lectins

Pinellia ternate and Pinellia pedatisecta lectins with biological functions could be obtained through procaryon expression. Their biological similarities and differences were analyzed and further experiments were conducted to discuss the agglutinative activity difference between polymer lectin and metamer lectin. The results showed that agglutinative activity of Pinellia pedatisecta was four times more than that of Pinellia ternate. The differences of the agglutinative activity and pharmacologic action were mainly because two site mutations of Pinellia pedatisecta in the third active site compared with the amino acids sequence in Pinellia ternate. In addition, the metamer lectin expressed by prokaryotic expression system did not agglutinate rabbit red blood cells. This research conduct further discussion about the difference between two lectins and the difference between polymer lectin and metamer lectin. It would be applicable for solving the problem of lacking Pinellia resources.