A simple and efficient method for DNA extraction from grapevine cultivars andVitis species |
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| Authors: | Muhammad A. Lodhi Guang-Ning Ye Norman F. Weeden Bruce I. Reisch |
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| Affiliation: | 1. Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, 14456, Geneva, NY, USA
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| Abstract: | A quick, simple, and reliable method for the extraction of DNA from grapevine species, hybrids, andAmpelopsis brevipedunculata (Vitaceae) has been developed. This method, based on that of Doyle and Doyle (1990), is a CTBA-based extraction procedure modified by the use of NaCl to remove polysaccharides and PVP to eliminate polyphenols during DNA purification. The method has also been used successfully for extraction of total DNA from other fruit species such as apple (Malus domestica), apricot (Prunus armeniaca), cherry (Prunus avium), peach (Prunus persica), plum (Prunus domestica), and raspberry (Rubus idaeus). DNA yield from this procedure is high (up to 1 mg/g of leaf tissue). DNA is completely digestible with restriction endonucleases and amplifiable in the polymerase chain reaction (PCR), indicating freedom from common contaminating compounds. |
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| Keywords: | DNA extraction Vitis polyphenols polysaccharides RAPD restriction digestion |
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