Site of synthesis and phylogenetic distribution of a hemolymph trophic factor of the tobacco hornworm,Manduca sexta |
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Authors: | J J Wielgus L B Aden R M Franks |
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Institution: | (1) Department of Biology, Washington and Lee University, 24450 Lexington, VA;(2) School of Medicine, Tulane University, 1430 Tulane Avenue, 70112 New Orleans, LA |
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Abstract: | Identification of fith instar larvalManduca sexta fat body and epidermis as sites of synthesis of a hemolymph protein (hemolymph trophic factor or HTF) was achieved using
in vitro3H-leucine incorporation into protein and subsequent immunoprecipitation of tissue homogenates. Fat body is the primary site
of HTF synthesis with a maximal rate on Day 1; epidermis is a secondary site with peak synthesis on Day 0. In vitro radiolabelling
followed by TCA precipitation of general protein of fat body and epidermal homogenates suggest that fat body actively elaborates
protein on Days 0–5 with peak rates on Days 1 and 4, while epidermis is active on Days 0–5 with a peak rate on Day 3. Based
on Anti-HTF ELISA estimates, HTF 500 to 1000 μg/ml] was found in the hemolymph of representatives of the insect orders Blattodea,
Hemiptera, Orthoptera, and Lepidoptera and in the class Crustacea, but not in the class Merostomata. These studies suggest
a possible fundamental role for HTF among modern arthropods in cuticular deposition involving both epidermis and fat body.
The physiological role of HTF is undetermined. |
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Keywords: | Manduca sexta hemolymph proteins immunoprecipitation ELISA fat body epidermis |
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