Express analysis of protein amino acid sequences. Primary structure of Penicillium chrysogenum 152A guanyl-specific ribonuclease |
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| Authors: | S V Shlyapnikov S I Bezborodova V A Kulikov G I Yakovlev |
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| Affiliation: | 1. Institute of Molecular Biology, the USSR Academy of Sciences, Vavilova 32, Moscow 117312, USSR;2. Institute of Genetics and Selection of Industrial Microorganisms, Vavilova 32, Moscow 117312, USSR |
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| Abstract: | The complete amino acid sequence of Penicillium chrysogenum 152A guanyl-specific RNase has been established using automated Edman degradation of two non-fractionated peptide mixtures produced by tryptic and staphylococcal protease digests of the protein. The RNase contains 102 amino acid residues: His2, Arg3, Asp7, Asn8, Thr5, Ser11, Glu4, Gln2, Pro4, Gly11, Ala13, Cys4, Val8, Ile3, Leu3, Tyr9, Phe5 (Mr 10 747). |
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