Isolation of Human Clq as a by-Product of Routine Fractionation |
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Authors: | C. Granier A. Faure R. Tavernier M. Steinbuch |
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Affiliation: | Department of Fractionation , Centre National de Transfusion Sanguine , Avenue des Tropiques, 91400, Orsay-Courtaboeuf, France |
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Abstract: | Clq can be isolated as a by-product of large scale fractionation. The euglobulins of fraction III are submitted to chromatography on CM-cellulose in a batch-procedure. The active fraction being frequently contaminated by lysozyme the protein can be further purified by gel-filtration in the presence of carbohydrates. The purified Clq is suitable for the detection of immune complexes. |
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Keywords: | guava solid-state fermentation tannase tannic acid |
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