Rapid Purification of Fluorescent Enzymes by Ultrafiltration |
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| Authors: | M. Aaron Benjaminson T. Satyanarayana |
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| Affiliation: | 1. North Star Research, Inc. , 198 Broadway, New York, New York, 10038;2. NASA Ames Research Center , Moffett Field, California, 94035 |
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| Abstract: | In order to expedite the preparation of fluorescently tagged enzymes for histo/cytochemistry, a previously developed method employing gel column purification was compared with a more rapid modern technique using the Millipore Immersible CX-ultrafilter. Microscopic evaluation of the resulting conjugates showed comparable products. Much time and effort is saved using the new technique. |
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