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The Preparation of Silica Gel Thin Layers with a New Apparatus |
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| Authors: | D. T. Organisciak J. D. Klingman W. A. Tanski |
| Affiliation: | 1. Department of Biochemistry , Wright State University School of Medicine , Dayton, Ohio, 45431;2. Department of Biochemistry , State University of New York at Buffalo , Buffalo, New York, 14214;3. Department of Technical Services , State University of New York at Buffalo , Buffalo, New York, 14214 |
| Abstract: | An apparatus for the preparation of uniform thin layer chromatography gel layers is described. The apparatus employs rubber cushion runners to compensate for differences in plate thickness and a gel applicator which functions independently of plate edge variability. Consistently uniform layers are prepared by careful establishment of the plate-to-applicator distance. Silica gel layers averaged 87% of the applied thickness with a variability of ±2% within a single run and ±8% between independent runs. |
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