An improved Method for Isolation of Rat Testis Golgi Apparatus H. H. Mollenhauer |
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| Authors: | S E Nyquist K Acuff Charles F Kettering |
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| Institution: | 1. Research Laboratory Yellow Springs , Ohio, 45387;2. Department of Botany and Plant Pathology , Purdue University , Lafayette, Indiana, 47907 |
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| Abstract: | The preparation of Golgi apparatus fractions from rat testis germ cells free from contamination by residual body fragments was accomplished by the use of the Yeda press as the homogenization device. The Golgi apparatus thus prepared retained excellent stuctural intactness. This method also allows for isolation of Golgi apparatus from single cell suspensions. |
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| Keywords: | α-amylase Bacillus detergent submerged fermentation (SmF) thermostable |
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