Phenol Extraction Op 28 S Ribosomal RNA Prom Rat Liver Cytoplasm |
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| Authors: | M D Dabeva |
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| Institution: | Institute of Biochemistry, Bulgarian Academy of Sciences , Sofia, Bulgaria |
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| Abstract: | A simple and reproducible phenol method for the isolation of 28 S ribosomal RNA from rat liver cytoplasm, free from poly(A)-RNA is described. The procedure is based on the observation that at lower pH of the homogenate (pH 5.5) 28 S ribosomal RNA is extracted, while 18 S ribosomal RNA remains in the interphase layer. Isolation of pure 28 S or 18 S ribosomal RNA in preparative amounts requires density gradient cen-trifugation or preparative gel electrophoresis. In this communication a rapid and reproducible method for the isolation of 28 S ribosomal RNA is proposed. |
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