Preparation and Study of a Fluorescent Sugar Analog Competitive Inhibitor of Yeast Hexokinase |
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| Authors: | Paul A. Lartey Moises Derechin |
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| Affiliation: | Department of Biochemistry , State University of New York at Buffalo , Buffalo, New York, 14214 |
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| Abstract: | Abstract The preparation of 2-deoxy-2-amino-N-(5-dimethylamino-l-naphthalene sulfonyl)-glucose (III) designed as a fluorescent competitive inhibitor of hexokinase was achieved after reacting 2-deoxy-2-aminoglucose and l-dimethylamino-5-naphthalene sulfonyl chloride. (III) showed fluorescence excitation and emission maxima in water at 330 and 507 nm, respectively. (III) was found to competitively inhibit hexokinase and a value of Ki = 3.0 × 10?3 M was obtained for the system hexokinase B + Mg. ATP + glucose at pH 8.4. |
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