Three Modifications of the Dodecyl Sulfate Method of Kay et al. for Isolating DNA from Calf Thymus |
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| Authors: | A L Dounce Regina Ickowicz David Volkman Richard Turk |
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| Institution: | The Biochemistry Department , University of Rochester School of Medicine and Dentistry , 260 Crittenden Blvd., Rochester, New York, 14642 |
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| Abstract: | Two rapid methods for the isolation of calf thymus DNA are described which are modifications of the method of Kay et al. 1 for isolating DNA. The principal changes introduced are that degelation of the DNA-nucleo-protein gels is accomplished rapidly by the use of mild shear or by pH adjustment in the presence of isopropanol instead of by the more time-consuming autolytic proteolysis used in the original method. |
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