Improved Purification and Some Molecular and Kinetic Properties of sn-Glycerol-3-Phosphate Dehydrogenase from Saccharomyces cerevisiae |
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| Authors: | Shiaw-Min Chen Mark W. Trumbore Joanne E. Osinchak Joseph R. Merkel |
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| Affiliation: | 1. Department of Chemistry , Lehigh University , Seeley G. Mudd Building 6, Bethlehem, PA, 18015;2. Department of Biochemistry , University of Connecticut Health Center , Farmington, CT, 06032 |
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| Abstract: | The purification procedure for isolating sn-glycerol-3-phosphate dehydrogenase (EC 1. 1. 1. 8) from Saccharomyces cerevisiaewas improved by the introduction of an ion-exchange step. Enzyme yields were doubled and the specific activity was increased as compared to the original procedure. A new value of 42, 000 was obtained for the molecular weight by several denaturing methods. By native gel chromatography the molecular weight appears to be 31, 000 as reported earlier. Michaelis constants were found to be 0. 37mM with dihydroxyacetone phosphate as the variable substrate and 0. 018mM for NADH as the variable substrate. |
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