Purification and Aggregation of Influenza Virus by Precipitation with Polyethylene Glycol |
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Authors: | A. Poison |
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Affiliation: | M. R. C. and U. C. T. Virus Research Unit, Medical School , Observatory, Cape Town |
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Abstract: | Influenza virus may be purified and rendered free of extraneous proteins by precipitation and aggregation with polyethylene glycol at polymer concentrations of 1 to 4%. The precipitated virus is superior antigenically to the virus in monomeric and in the ether dissociated forms. When the virus is precipitated at polyethylene glycol concentrations of 5% and higher the virus is not aggregated and is associated with extraneous protein which co-precipitates with the infectious agent. |
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