Purification Of Methionine Acceptor tRNA On Arginine-Agarose |
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| Authors: | Francis T. Jay D. S. Jones |
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| Affiliation: | Department of Biochemistry , University of Liveroool , P. 0. Box 1V7, Liverpool, L69 3BX, U. K. |
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| Abstract: | Crude E. coli tRNA or enriched methionine acceptor tRNA can be separated into three stiecies on a column of arginine-agarose. The first peak eluted is tRNAMet and the latter two peaks are two forms of tRNAMet f. From crude tRNA, tRNAMet m is obtained in approximately 50% purity. Arginine-agarose separates enriched methionine accepting tRNA into three homogeneous fractions. |
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