Large Scale Purification of Alpha-1 Trypsin Inhibitor from Human Plasma |
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Authors: | Lawrence F. Kress M. Laskowski Sr. |
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Affiliation: | The Laboratory of Enzymology , Roswell Park Memorial Institute , Buffalo, N. Y., 14203 |
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Abstract: | A convenient large scale purification of the alpha-1 trypsin inhibitor from human plasma was achieved by conventional salting out and column chromatography methods. The pure inhibitor complexes 0.68 μgs of bovine trypsin per ug inhibitor, and 0.60 μgs human trypsin per μg inhibitor. The inhibitor is homogeneous by equilibrium chromatography, disc electrophoresis, and ultracentrifugation. Conditions for its storage with minimal loss of activity are described. The advantages of the present preparation are higher potency, long-term stability, and large scale. |
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Keywords: | Amazonian fruits eco-friendly bio-processes estrogen bioremediation ligninolytic enzymes |
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