Isolation of Components from the Low-Sulphur Proteins of Wool by Fractional Precipitation

The two major components of the low-sulphur S-carboxymethylkerateine fraction from wool (components 7 and 8) have been isolated by methods involving fractional precipitation from n-propanol/water and from 4 M LiBr. The materials obtained in this way are similar in amino acid composition and in gel electrophoresis properties to fractions obtained by column chromatographic techniques and are of at least equal purity. The procedures are relatively quick and can be scaled up.