Removal of Unwanted Proteins prom Cell Extracts by Means of Antiserum |
| |
| Authors: | Berit Lundqvist G. Bertani |
| |
| Affiliation: | Microbial Genetics Laboratory , Karolinska Institutet , 104 01, Stockholm, Sweden |
| |
| Abstract: | The efficiency of removal of soluble proteins in cell-free bacterial extracts by means of antiserum from rabbits immunized with similar extracts vas measured. Precipitation followed by Sephadex gel-chromatography was used. Up to 807percnt; (exceptionally 907percnt;) removal could be obtained. The method might be applied to enrichment for “foreign” cell-extract components, for example, viral products in virus infected cells. Tests for the specificity of the method are also presented. |
| |
| Keywords: | |
|
|
