A Simple,Rapid, High Yield Isolation and Purification Procedure for Chloroperoxidase Isoenzymes |
| |
| Authors: | E Gonzalez-vergara D C Ales H M Goff |
| |
| Institution: | Department of Chemistry , University of Iowa , Iowa City, Iowa, 52242, U.S.A. |
| |
| Abstract: | A simple four-step procedure has been developed for Isolation of chloroperoxldase from the mold Caldarlomyces fumago. Polyethyleneglycol precipitation of the contaminating pigment in the growth medium, followed by chromatography of the soluble enzyme fraction on a QAE-ZetaPrep-250 cartridge and ammonium sulfate precipitation affords Isolation of the chloroperoxldase. Extensive dialysis and chromatography on DE-53 cellulose allows the separation and further purification of chloroperoxldase A and B isoenzymes. |
| |
| Keywords: | |
|
|
