Large Scale Isolation of Pig Muscle Phosphoglucose Isomerase |
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| Authors: | Danny M Gee Richard H Palmieri Gerald G Porter Ernst A Noltmann |
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| Institution: | 1. Department of Biochemistry and Division of Biomedical Sciences , University of California , Riverside, California, 92521;2. Neurobiology Laboratory , Andrus Gerontology Center, University of Southern California , Los Angeles, CA, 90007 |
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| Abstract: | large-scale purification procedure for phosphoglucose isomerase from pig skeletal muscle is described. It consists of two fractionations by selective precipitation and two ion exchange chromatography steps yielding an end product of approximately 900 units (micromoles of sub-strate converted to product per rain per mg of protein, at 30°) specific activity. The method separates three isoenzymic forms with an overall recovery of about 30% of the original total enzyme activity in the form of Isoenzyme III, the latter being the predominant enzyme species. |
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