Mobility-concentration effects for RNA in agar gel electrophoresis |
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| Authors: | D Staynov |
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| Affiliation: | 1. UCL Institute for Sustainable Heritage, University College London, 14 Upper Woburn Place, London, WC1H 0NN, UK;2. Faculty of Chemistry and Chemical Technology, University of Ljubljana, Večna pot 113, 1000, Ljubljana, Slovenia;3. Institute of Applied Physics “Nello Carrara” IFAC, Italian National Research Council CNR, Via Madonna del Piano 10, 50019, Sesto Fiorentino, FI, Italy;4. Conservation Department, Faculty of Archaeology, Fayoum University, Fayoum, Egypt;1. Global Food Studies, University of Adelaide, Adelaide, SA, Australia;2. School of Commerce, University of South Australia, Adelaide, SA, Australia;1. River Consulting, Townsville, Queensland 4810, Australia;2. The Cairns Institute and Division of Tropical Environments and Societies, James Cook University, Townsville, Australia;3. Earth-to-Ocean Consulting, Townsville, Australia;4. ARC Centre for Excellence in Coral Reef Research, James Cook University, Townsville, Australia;5. Queensland Government Department of Science, Information Technology and Innovation Brisbane, Australia;1. Mellen Center for MS Treatment and Research, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA;2. Atlas5D, Inc. One Broadway, Cambridge, MA 02142, USA;3. Institute for Technology Assessment, 101 Merrimac St., Boston, MA 02114, USA |
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| Abstract: | The dependence of the mobility of RNA species on their concentration in agar gel electrophoresis is explained in terms of adsorption on the gel matrix. The number of interaction sites on RNA molecules depends on the origin of the RNA and on the extent of deproteinization. The ratio of sites on the RNA of the large and small ribosomal subunits is equal to the ratio of their molecular weights for both rat liver and E. coli. The results suggest that the interactions with the gel are due to some impurity, most probably protein. |
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