Cloning of Clostridium thermocellum endoglucanase genes in Escherichia coli |
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| Authors: | B K Bumazkin G A Velikodvorskaya K Tuka M A Mogutov |
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| Affiliation: | CENDISI Bioprocess, USSR Academy of Sciences, Moscow. |
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| Abstract: | Six independent and distinct cel genes coding endoglucanases have been selected from C. thermocellum pUC19-based gene bank in E. coli TG1. E. coli-derived Cel-proteins possessing Mr from 39,000 to 61,000 are able to cleave lichenan, as well as xylan and carboxymethyl cellulose. Cel 7- and Cel 8-endoglucanases are characterized by cellobiohydrolase type substrate specificity, being able to cleave model fluorogenic aryldisaccharide substrate MU-G2. The clone pCU110 (cel 7) produces about 10-fold more endoglucanase activity than other clones. |
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