The Osmotic-1 Locus of Neurospora crassa Encodes a Putative Histidine Kinase Similar to Osmosensors of Bacteria and Yeast |
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Authors: | Marc M Schumacher Carol S Enderlin Claude P Selitrennikoff |
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Institution: | (1) University of Colorado Health Sciences Center, B-111, 4200 East 9th Avenue, Denver, CO 80262, USA , US;(2) University of Colorado Health Sciences Center and MycoTox, Inc., B-111, 4200 East 9th Avenue, Denver, CO 80262, USA , US |
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Abstract: | Osmotically sensitive mutants of Neurospora crassa
are unable to grow on medium supplemented with 4% NaCl, have altered
morphologies and cell-wall compositions, and are resistant to dicarboximide
fungicides. Osmotic-1 (os-1) mutants have a unique characteristic of
forming protoplasts that grow and divide in specialized liquid medium,
suggesting that the os-1
+ gene product is important for
cell-wall assembly. A cosmid containing the os-1
+ locus of
N. crassa, isolated from a genomic cosmid library by
chromosomal walk from a closely linked gene, was used to subclone the
os-1
+ gene by functional complementation of an
os-1 mutant. Analysis of the sequence of complementing DNA predicts
that os-1
+ encodes a predicted protein similar to
sensor-histidine kinases of bacteria and a yeast osmosensor-histidine kinase.
Importantly, the predicted os-1
+ protein is identical to
the N. crassa nik-1 predicted protein that was identified by
using polymerase chain reaction primers directed against histidine kinase
consensus DNA sequences. Our results indicate that nik-1 and
os-1 encode the same osmosensing histidine kinase that plays an
important role in the regulation of cell-wall assembly and, probably, other
cell responses to changes in external osmolarity.
Received: 12 September 1996 / Accepted: 12 November 1996 |
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Keywords: | |
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