On the photosynthetic properties of marine bacterium COL2P belonging to Roseobacter clade |
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Authors: | Michal Koblížek Jarmila Mlčoušková Zbigniew Kolber Jiří Kopecký |
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Institution: | 1. Institute of Microbiology CAS, Opatovicky mlyn, 379 81, T?eboň, Czech Republic 2. Institute of Physical Biology, University of South Bohemia, Zámek 36, 373 33, Nové Hrady, Czech Republic 3. Monterey Bay Aquarium Research Institute, 7700 Sandholdt Road, Moss Landing, CA, 93901, USA
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Abstract: | Aerobic anoxygenic phototrophs (AAPs) are prokaryotic microorganisms capable of harvesting light using bacteriochlorophyll-based
reaction centres. Marine AAP communities are generally dominated by species belonging to the Roseobacter clade. For this reason, we used marine Roseobacter-related strain COL2P as a model organism to characterize its photosynthetic apparatus, level of pigmentation and expression
of photosynthetic complexes. This strain contained functional photosynthetic reaction centres with bacteriochlorophyll a and spheroidenone as the main light-harvesting pigments, but the expression of the photosynthetic apparatus was significantly
reduced when compared to truly photoautotrophic species. Moreover, the absence of peripheral light-harvesting complexes largely
reduced its light-harvesting capacity. The size of the photosynthetic unit was limited to 35.4 ± 1.0 BChl a molecules supplemented by the same number of spheroidenone molecules. The contribution of oxidative phosphorylation and photophosphorylation
was analysed by respiration and fluorometric measurements. Our results indicate that even with a such reduced photosynthetic
apparatus, photophosphorylation provides up to three times higher electron fluxes than aerobic respiration. These results
suggest that light-derived energy can provide a substantial fraction of COL2P metabolic needs. |
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