|
|||||
|
|
Expression of tobacco mosaic virus RNA in transgenic plants |
|
| Authors: | Jun Yamaya Masaharu Yoshioka Tetsuo Meshi Yoshimi Okada Takeshi Ohno |
| Affiliation: | (1) Plant Laboratory, Kirin Brewery Co., Kitsuregawa-machi, Shioya-gun, 329-14 Tochigi, Japan;(2) Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Hongo, 113 Tokyo, Japan;(3) Department of Agricultural Chemistry, Tokyo University of Agriculture and Technology, 183 Fuchu, Tokyo, Japan |
| Abstract: | Summary Tobacco mosaic virus (TMV) is a message-sense, single-stranded RNA virus that infects many Solanaceae plants. A full-length cDNA copy of TMV genomic RNA was constructed and introduced into the genomic DNA of tobacco plants using a disarmed Ti plasmid vector. Transformed plants showed typical symptoms of TMV infection, and their leaves contained infectious TMV particles. This is the first example of the expression of RNA virus genomic RNAs in planta. |
| Keywords: | Tobacco mosaic virus Transgenic plant In planta expression Ti plasmid |
| 本文献已被 SpringerLink 等数据库收录！ | |