(1) Department of Biological Science and Technology, Science University of Tokyo, 278 Noda, Chiba, Japan;(2) Department of Industrial Chemistry, Faculty of Engineering, The University of Tokyo, 113 Bunkyo-ku, Tokyo, Japan
Abstract:
Summary Two sets of the Shine-Dalgarno sequence and the initiation codon (ATG) for translation of a gene encoding the protein SSI (Streptomyces subtilisin inhibitor) were studied in vivo by site-directed mutagenesis. The result shows that each ATG can function as an initiator of translation in either Streptomyces lividans 66 or Escherichia coli. The choice of initiation codon seems dependent on the host strain and is closely related to the processing mechanism of pre-SSI protein. The upstream ATG is presumed to be utilized preferentially giving two cleavage sites in pre-SSI in S. albogriseolus S-3253, the original SSI producer strain.Abbreviations SD
Shine-Dalgarno
- SSI
Streptomyces subtilisin inhibitor