Characterization of folic acid-PAMAM conjugates: drug loading efficacy and dendrimer morphology |
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| Authors: | P Chanphai |
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| Institution: | Department of Chemistry-Biochemistry and Physics, University of Québec at Trois-Rivières, C. P. 500, Trois-Rivières, Quebec G9A 5H7, Canada |
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| Abstract: | We report the loading efficacy of folic acid (FA) by polyamidoamine (PAMAM-G3 and PAMAM-G4) nanoparticles in aqueous solution at physiological pH. Thermodynamic parameters ΔH = ?47.57 (kJ Mol?1), ΔS = ?122.78 (J Mol?1, K?1) and ΔG = ?10.96 (kJ Mol?1) showed FA-PAMAM bindings occur via H-bonding and van der Waals contacts. The stability of acid-PAMAM conjugate increased as polymer size increased. The acid loading efficacy was 40 to 50%. TEM images exhibited major polymer morphological changes upon acid encapsulation. PAMAM dendrimers are capable of FA delivery in vitro. |
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| Keywords: | folic acid dendrimer conjugation loading efficacy thermodynamic TEM modeling |
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