Circadian PER2 protein oscillations do not persist in cycloheximide-treated mouse embryonic fibroblasts in culture |
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Authors: | Motomi Tainaka Yuichi Inoue Iori Murai Hitoshi Okamura |
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Institution: | Department of Systems Biology, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan |
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Abstract: | It is not known whether the endogenous mammalian core clock proteins sustain measurable oscillations in cells in culture where de novo translation is pharmacologically inhibited. We studied here the mammalian core clock protein PER2, which undergoes robust circadian oscillations in both abundance and phosphorylation. With a newly developed antibody that enables tracing the endogenous PER2 protein oscillations over circadian cycles with cultured mouse embryonic fibroblast cells, we provide evidence that PER2 does not persist noticeable circadian rhythms when translation is inhibited. |
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Keywords: | PER2 clock protein antibody cycloheximide |
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