Enzyme assay in cultures of Escherichia coli by a continuous flow method based on lysis from without by a phage ghost. |
| |
Authors: | C T Ou I Matsumoto J Rozhin T T Tchen |
| |
Affiliation: | Department of Chemistry, Wayne State University, Detroit, Michigan 48202 USA |
| |
Abstract: | Lysis of Escherichia coli from without by excess of phage ghost has been shown to give excellent yield of several enzymes. The application of lysis from without to a continuous determination of enzymes in growing cultures of E. coli is illustrated with β-galactosidase. This application can be used in studies on changes of a large number of enzymes during metabolic perturbation (induction, repression, etc.) of growing cultures of E. coli. |
| |
Keywords: | Author to whom all correspondence should be mailed. |
本文献已被 ScienceDirect 等数据库收录! |