CTLA-4+49A>G基因多态性与结直肠癌的相关性研究

目的:探讨细胞毒性T淋巴细胞相关抗原-4(CTLA-4)+49 AG位点多态性与结直肠癌发生的相关性,为早期预测结直肠癌的发生提供临床参考依据。方法:选取结直肠癌病例231例未实验组和正常健康体检者325例为对照组,取其空腹外周静脉血提取DNA后,采用聚合酶链式反应(PCR)技术对CTLA-4基因第1外显子区+49位点DNA进行扩增,产物用限制性片段长度多态性(RFLP)方法检测CTLA-4第1外显子区+49AG位点的多态性,比较两组杂合子AG和纯合子AA、GG基因型发生的频率、A等位基因与G等位基因的分布,分析CTLA-4+49 AG位点多态性与结直肠癌发生的相关性。结果:两组间CTLA-4基因外显子1区+49AG位点杂合子AG和纯合子AA、GG基因型发生的频率以及A等位基因与G等位基因的分布比较差异均无统计学意义(P0.05)。结论:CTLA-4基因外显子1区+49 AG基因多态性与我省汉族人群结直肠癌的发病无显著相关性。

Correlative Study on CTLA-4+49A>G Polymorphism Gene Polymorphism and Colorectal Cancer

Objective:To study the relation of CTLA-4 +49 A>G polymorphism on thesusceptibility of colorectal cancer, and provide clinical help for the earlyprediction and monitoring of colorectal cancer.Methods:Selection of colorectal cancer in 231 cases; 325 cases ofnormal control group, according to kit instructions after extracting DNA, using polymerase chain reaction CTLA-4 gene first exon region amplification of the +49 locus DNA, products with restriction fragment length polymorphism (restrictionfragment length polymorphism, RFLP) exon region of +49A>Gpolymorphism detection of CTLA-4 first, a comparative analysis of nodeand control groups in rectal cancer, to analyze the correlation between CTLA-4 +49 A>G polymorphism and the risk of colorectal cancer for further.Results:Two groups of CTLA-4 gene exon 1 +49A>G locus, heterozygous andhomozygous AG AA, GG genotype frequency showed not statistically significant difference between two groups (P>0.05); A alleles and G alleles between the two groups were not significantly different (P>0. 05).Conclusion:There was no association between the prevalence of CTLA-4 gene exon 1 region of +49 A>G gene polymorphism and people of our province of colorectal cancer.